My blood group is A- (negative) and I had anti-D with my first pregnancy. Will I have it again with my second?

Most probably yes, as if the baby is blood group rhesus positive (+) then you might produce harmful antibodies, which anti-D effectively neatralizes if given early enough. The only situation in which you might decide not to have anti-D is if the father of your baby has had his blood group checked and is himself rhesus negative. In this case, your baby would be rhesus negative and you wouldn't need anti-D.
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